An anaerobic mitochondrion that produces hydrogen

Lysozyme, a mediator of sepsis that produces vasodilation by hydrogen peroxide signaling in an arterial preparation.

In septic shock, systemic vasodilation and myocardial depression contribute to the systemic hypotension observed. Both components can be attributed to the effects of mediators that are released as part of the inflammatory response. We previously found that lysozyme (Lzm-S), released from leukocytes, contributed to the myocardial depression that develops in a canine model of septic shock. Lzm-S ...

Overproduction of Hydrogen from an Anaerobic Bacterium

A derivative of the anaerobic bacterium Clostridium phytofermentans was isolated and shown to produce two moles of hydrogen per mole of glucose and similar yields from cellulosic feedstocks. The strain (cpnit-1) was selected for its rapid growth in a chemostat under nitrogen fixation conditions. The rare ability of cpnit-1 to stably, anaerobically produce hydrogen while rapidly fixing its own n...

Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide.

In this direct colorimetric procedure, serum triglycerides are hydrolyzed by lipase, and the released glycerol is assayed in a reaction catalyzed by glycerol kinase and L-alpha-glycerol-phosphate oxidase in a system that generates hydrogen peroxide. The hydrogen peroxide is monitored in the presence of horseradish peroxidase with 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone as th...

Nourishment Essential Requirement for Bio-Hydrogen Production in Anaerobic Condition

Oxidative inactivation of rhodanese by hydrogen peroxide produces states that show differential reactivation.

Controlled conditions have been found that give complete reactivation and long term stabilization of rhodanese (EC 2.8.1.1) after oxidative inactivation by hydrogen peroxide. Inactivated rhodanese was completely reactivated by reductants such as thioglycolic acid (TGA) (100 mM) and dithiothreitol (DTT) (100 mM) or the substrate thiosulfate (100 mM) if these reagents were added soon after inacti...